Analysis of clinical cases by conventional cytogenetics, fluorescence in situ hybridization and nested PCR revealed that H3K4me3, H3K9ac and H3K27ac were significantly enriched in the EWSR1 promoter in Ewing sarcoma to facilitate transcription [93,94].The transcription factor SP1 directly binds to the EWSR1::FLI1 promoter to trigger transcription, a process induced by activated PI3K/Akt signaling [95]. This evidence concerns the gene EWSR1 and Ewing sarcoma.