Moreover, a microarray analysis in Ewing sarcoma A673 cells revealed that depletion of endogenous EWSR1::FLI1 by retroviral siRNAs upregulated 320 genes and downregulated 1151 genes, among which NKX2.2 was reported as a critical EWSR1-FLI1 downstream target [65]. The gene discussed is EWSR1; the disease is Ewing sarcoma.