Conversely, the regulatory sequences of downregulated Ide and Nicastrin showed enhanced binding of epigenetic repressor histone deacetylase 2 (HDAC2) and reduced levels of H3K9ac in the hippocampi of HFD offspring (Ide: +203.4% for HDAC2, p = 5.26 × 10−5, −49.9% for H3K9ac, p = 0.0013; Nicastrin: −72.7% for H3K9ac, p = 6.85 × 10−6; n = 6; Figure 4C), indicating that epigenetic derangement of AD-related gene promoters may be involved in the maternal HFD-dependent worsening of AD phenotype. Here, NCSTN is linked to Alzheimer disease.