This immunohistochemical appearance of reciprocation (Figure 6A–C) between CD44 and MMP9 [39,56,57,58,59,60] was not expected to exhibit in the FFPE-ESCC sample and its significance remains to be clarified in future experiments where we can investigate an underlying mechanism for invasion and matrix remodeling expression interplay in a DEM sample to simulate the clinical tissue. The gene discussed is MMP9; the disease is esophageal squamous cell carcinoma.