ACTA1 and prostate cancer: In support, histopathological analysis and immunohistochemistry (IHC) staining of 40 prostate cancer patient samples revealed that the presence of a reactive stromal (characterized by expansion and thickening of collagen fibers and significant reduction of smooth muscle fibers), correlated with a decrease in αSMA-expressing cells and increased numbers of vimentin-, IGF-1-, MMP-2- and FGF-2-positive cells, that label CAFs [152].