CHRNE and mastitis: Risseti et al. (2017) investigated 71 T. pyogenes strains recovered from mastitis (n =35), and non-mastitis and reported the presence of plo (100.00%), fimA (98.6%), nanP (78.9%), fimE (74.6%), fimC (64.8%), nanH (63.4%), cbpA (8.4%) and fimG (5.6%) in their studied strains [9].The variability in gene content is the basis of bacterial evolution, and gene truncation is significant for shaping bacterial genomes [77].