To evaluate the effect of IL-1β blockade in the urethane-induced LC model, we treated WT and Il1b KO mice i.p with urethane and i.n with HDM or VEH following the protocol shown in Fig. 1A. In line with the effect of IL-1β neutralization in KrasG12D mice, the tumor multiplicity and the tumor area were strongly reduced in Il1b KO mice treated with urethane and HDM as compared to WT mice that received the same treatments and were not significantly different from the tumor multiplicity and tumor area of Il1b KO mice treated with urethane and VEH (Fig. 5D, F–G). The gene discussed is IL1B; the disease is laryngotracheoesophageal cleft.