This was validated by ISH for Ccnd1 mRNA in tissue sections of 9 months old HdhQ200 mice (Fig. 4A), showing a 1.7-fold increase in Ccnd1 staining in the granule layer in HD mice (Wilcoxon rank sum test, p = 0.002) (Fig. 4B). The gene discussed is CCND1; the disease is Huntington disease.