We first detected BACH1, SLC7A11, GPX4 and ACSL4 protein expression and the sensitivity to the ferroptosis inducer RSL3 in six ESCC cell lines (KYSE150, KYSE170, KYSE180, KYSE30, KYSE410 and KYSE510) (Fig. 3A, B). This evidence concerns the gene SLC7A11 and esophageal squamous cell carcinoma.