Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and gene set enrichment analysis (GSEA) of the RNA-seq data indicated that TRIM15 was associated with multiple cancer-related pathways, including pathways in cancer, the PI3K-AKT signaling pathway, the MAPK signaling pathway, the mTOR signaling pathway, the TNF signaling pathway, the HIF-1 signaling pathway, and the EGFR kinase inhibitor resistance pathway (Fig. 4b, c). The gene discussed is TNF; the disease is cancer.