Infection of THP-1 macrophages with P. aeruginosa PAO1 (but not with ΔlasB) resulted in highlevels of LasB activity in the supernatant, as measured by hydrolysisof Sub115, and correspondingly high levels of activated IL-1β,as measured by generation of a robust signal in the IL-1 reporterassay. Here, IL1B is linked to infection.