To further determine the specific M1 or M2 macrophages related to the expression of PD-L1 or PD-L2 during the infection of C. hepatica, the macrophages were collected from the livers of the infected mice and the expressions of PD-L1 and PD-L2 were measured on the macrophages gated with F4/80+CD86+ (M1) or F4/80+ CD206+ (M2). This evidence concerns the gene CD86 and infection.