In other studies, TBT caused irregular estrous cycles, disturbed ovarian development, including increased presence of atretic and cystic follicles, fewer CLs, antral follicles and increased levels of atretic follicles, hyperandrogenism, high levels of serum LH, and decreased levels of serum sex hormone-binding globulin, in addition to an increase in the RXR/PPAR signaling pathway and other proteins that are involved in androgen biosynthesis [65,69]. This evidence concerns the gene SHBG and hyperandrogenism.