In order to establish whether this poor endogenous NLRP6 expression was also true for other cell types, we performed western-blot analyses on a variety of cell types: two other human colon cancer cell lines (SW480 and HT29), the human embryonic kidney cell line HEK293T, human normal fetal colonic cells (CCD841), human normal adult colonic epithelial cells (HPCECs), the human adenocarcinoma of esophageal gastric junction cell line OE19, the human epidermal carcinoma cell line A431, human primary epidermal keratinocytes (NHEKs K23FC), and human primary dermal fibroblasts (NHDFs F6MC1). This evidence concerns the gene NLRP6 and colonic neoplasm.