ARPC5L and melanoma: To explore the role of the two ArpC5 isoforms in lamellipodia architecture and cell migration, we used a CRISPR-Cas9 knockout (KO) approach (42) to generate B16-F1 mouse melanoma cells lacking either ArpC5 (referred to as C5KO), ArpC5L (C5LKO), or both isoforms (C5/C5LKO).