The glioblastoma sample in the blot (Fig. 2; GBM) corresponds to spot A1 in Fig. 3, such as other samples are shown in dot blot: astrocytoma (Fig. 2; AST) in spot B2, oligodendroglioma (Fig. 2; ODG) in spot C4, and meningioma (Fig. 2; MNG) in spot D4 in Fig. 3.Purified bovine pyruvate carboxylase and lysate of hepatocarcinoma cells (Fig. 2) were used as positive controls. Here, PC is linked to glioblastoma.