The importance of this study are that (1) as reported, we showed that DLI is a viable salvage option for AML patients relapsed after alloSCT, (2) G-CSF mobilized DLI yields a prognosis similar to that of conventional DLI, and (3) cryopreservation of graft content does not negatively affect the outcomes of DLI. This evidence concerns the gene CSF3 and acute myeloid leukemia.