A random forest analysis showed that the TNS1 gene was the top contributor to this survival classification of ESCC; TNS1 was predominantly expressed in fibroblasts, and patients with a higher proportion of TNS1-positive fibroblasts in the tumor stroma have a poor prognosis and display lower CD8-positive T cell infiltration in the tumor parenchyma, associated with an immune exclusion phenotype [50]. The gene discussed is TNS1; the disease is esophageal squamous cell carcinoma.