ESC-410 and KYSE-150 ESCC cell lines were selected and divided into three groups: the control group (sh-NC), silenced-OIP5 gene group (sh-OIP5), and silenced-OIP5 group with an exogenous overexpression of OIP5 (sh-OIP5 + OIP5). The gene discussed is OIP5; the disease is esophageal squamous cell carcinoma.