ACSL1 and esophageal squamous cell carcinoma: Secondly, to further demonstrate the relationship between ACSL1 and OIP5, we selected the same ESCC cell lines and divided them into four groups including control group (sh-NC), silenced-ACSL1 gene group (sh-ACSL1), silenced-ACSL1 gene + overexpressing-ACSL1 genome (Sh-ACSL1 + ACSL1), and silenced-ACSL1 gene + OIP5-overexpression group (Sh-ACSL1 + OIP5).