By utilizing mass spectrometry analysis of metabolic intermediates in lipid metabolism-pathway (such as PLs and TGs) content assay and fluorescent staining of neutral lipids, we found that in the ESC-410 and KYSE-150 cell lines, the TGs and PLs content of the sh-ACSL1 group were significantly reduced compared with the control group, while in the silenced-ACSL1 gene + exogenous overexpressing-ACSL1 genome (Sh-ACSL1 + ACSL1), silencing the ACSL1 gene + exogenous overexpressing-OIP5 gene (Sh-ACSL1 + OIP5) restored TGs and PLs in ESCC cell lines to a certain level (Figure 6A,B). This evidence concerns the gene ACSL1 and esophageal squamous cell carcinoma.