When metformin was pretreated, cisplatin caused a change in the expression of genes involved in transcriptional misregulation in cancer, microRNAs in cancer, PI3K-Akt signaling pathway, axon guidance, Rap1 signaling pathway, serotonergic synapse, focal adhesion, cytokine–cytokine receptor interaction, inflammatory mediator regulation of TRP channel, serotonergic synapse, metabolic pathways, p53 signaling pathway, pathways in cancer, tight junction and so on in 24 h metformin-pretreated group (Table 1, Table S3). This evidence concerns the gene TP53 and cancer.