On the basis, taking advantage of the lack of function of the P53 gene in a variety of tumors, Kovacs et al. proposed a clever idea based on a therapeutic strategy that stimulates the function of P53, prepared Ag nanoparticles, and tested their cytotoxic effect on the osteosarcoma cells (U20S, Saos-2) which lack the function of P53 tumor suppressor gene (Kovacs et al., 2016). This evidence concerns the gene TP53 and osteosarcoma.