To discover the genetic alteration of TP53 in ESCC, we collected tumor samples to set up two cohorts, including 316 patients for sequencing analysis (276 patients out of 316 patients performed IHC to detect the expression of p53 protein), and 6,028 patients for the expression of p53 protein in TMA, for investigation in this study (Figure 1). Here, TP53 is linked to esophageal squamous cell carcinoma.