The reduction of parasite infection in MAPK4-KO cells was assessed to be minor due to the smaller scale using 96-well plates, but the infection rate was significantly reduced in MAPK4-KO and Mock-transfected MAPK4-KO cells, compared to both WT HCT-8 cells and MAPK4-KO HCT-8 cells transiently overexpressing MAPK4. The gene discussed is MAPK4; the disease is parasitic infectious disease.