The cGAS-STING pathway is crucial for IFN-β production by activating IRF3/7.55,56 In addition to various cGAS-STING agonists, physical irradiation is also explored for tumor immunotherapy by inducing the cGAS-STING activation.57 In this study, we found that chemotherapeutic drugs can act as cGAS-STING agonists through the lysosomal-nuclear pathway. The gene discussed is IFNB1; the disease is neoplasm.