This effect was associated with increased levels of RIP3 and a reduced number of anti-microbial Paneth cells, alluding to Caspase-8 regulation of necroptosis and inflammation in the pathogenesis of colitis.24 However, no changes in necroptotic cell death markers such as RIPK1, RIP3, or phospho-MLKL were observed in our study in the control or Caspase-8 inhibitor group. This evidence concerns the gene RIPK3 and colitis.