Animals exposed to both HFD and L-NAME manifested signs of heart failure supported by the NT-proBNP level and lung weight (LW) (wet/dry), indicative of pulmonary congestion, and we observed that these two indicators were modified in the ADRQβ-004 vaccine group (ADRQβ-004: 1211 ± 157 pg/mL versus HFD + L-NAME: 1447 ± 142 pg/mL, P = 0.009; ADRQβ-004: 8.9 ± 1.2 versus HFD + L-NAME: 11.2 ± 2.3, P = 0.038, respectively) (Fig. 3f, g). The gene discussed is NPPB; the disease is heart failure.