To prove this hypothesis, we initially generated two liver cancer cell lines stably expressing STAT3 short hairpin RNA (shRNA) or control shRNA using a lentiviral transduction system (Figure 1A and B, Figure 1—figure supplement 1A and B) and assessed the effect of STAT3 depletion on the expression of Pol III products by RT-quantitative PCR (qPCR). Here, STAT3 is linked to liver cancer.