INS and diabetes mellitus: In the Diabetes Prevention Type 1 (DPT-1) trial testing oral insulin to induce tolerance, protection was not significant when comparing all patients in treatment and placebo groups, but became significant when only patients with anti-insulin AAbs above 80 nU/mL were considered.24 This suggests that patients who have a more pronounced AAb reactivity to an antigen may be more responsive to this antigen in ASIT.