Recently, a method combining DNA-barcode-labeled MHC tetramers, CITE-seq and TCR-seq (termed TetTCR-SeqHD) was employed to profile CD8+ T cells in control and T1D individuals.65 While this approach provides a much deeper profiling of these rare T cells at the clonal level (thanks to TCR sequences), it is challenging to apply on a large scale (broad antigen-coverage by MHC multimers and large number of patients for screening purposes). The gene discussed is HLA-C; the disease is type 1 diabetes mellitus.