Parallel experiments in which HeLa cells were stained with a Vps34 antibody showed Vps34 localizing to WT and inlB-complemented ΔinlB LCVs as early as 10 min after infection, while remaining absent from ΔinlB LCVs (Fig. 4A, right, Fig. 4C, Fig. S5B). This evidence concerns the gene PIK3C3 and infection.