Rab5 was inhibited in HeLa cells prior to infection either by transfection with Rab5-S34N, a dominant-negative mutant in which the GTPase is locked in GDP-bound inactive form (47), or by using an siRNA pool targeting Rab5 isoforms A, B, and C (Fig. 6J and Fig. S9A). Here, RAB5A is linked to infection.