Further, shRNA-mediated knockdown of Smarcd3 in MSI2+ KPf/fC cells almost completely blocked flank tumor growth in NOD-SCID mice in vivo, reducing growth rate by over 4-fold, and total tumor cell and MSI2+ tumor stem cell counts by 2.5 and 3.5-fold (Fig. 1j, Supplementary Fig. 1i, j). This evidence concerns the gene MSI2 and neoplasm.