This placenta-on-a-chip 3D microfluidic platform was used to investigate (i) trophoblast–endothelial cell interactions during placental development, (ii) endothelial cell network formation and (iii) FKBPL and Gal-3 mechanisms during these processes, particularly within inflammatory conditions, which are characteristic of preeclampsia. Here, FKBPL is linked to preeclampsia.