Because there are some AA differences between TKTμ and M.tb TKT, we designed two additional M.tb TKT-peptide homologs (TKT1 and TKT3) to develop a novel direct ELISA and to quantify the levels of IgG against these three TKT peptides (TKTμ, M.tb TKT1, and M.tb TKT3) for the serodiagnosis of TB. This evidence concerns the gene TKT and tuberculosis.