The relative luciferase activity in LN229 glioma cells was markedly decreased after cotransfection of HIC2 and WT‐PTPRN2‐luc or HIC2 and Mut‐E2‐luc, while luciferase activity was not affected after cotransfection of HIC2 and Mut‐E1‐luc (Figure 6F). The gene discussed is HIC2; the disease is central nervous system cancer.