Western blotting of tumor samples showed that the combination treatment with CQ increased heat treatment induced the accumulation of LC3-II and cleaved-caspase 9, and retarded heat treatment induced the up-regulation of TGF-β2, Smad2 phosphorylation and N-cadherin and down-regulation of E-cadherin (Fig. 6D). The gene discussed is SMAD2; the disease is neoplasm.