In the distal colon, increased gene expression of zo‐1, ocln, cldn1, cldn2, and cldn5 was observed in the CRS group compared with the CON group, and prebiotics administration during CRS decreased the gene expression of ocln, cldn1, cldn2, cldn5, and cldn8 compared with the CRS group (Figure 3B). The gene discussed is CLDN8; the disease is congenital rubella syndrome.