EA reversed the elevated GnRH level and the protein and mRNA expressions of Kiss1 and Kiss1r. This indicated that the change of kisspeptin-GnRH/LH neural circuit is similar in different PCOS models, and EA could ameliorate the hyperactive GnRH/LH pulse, possibly by inhibiting the kisspeptin/ Kiss1r system. The gene discussed is GNRH1; the disease is polycystic ovary syndrome.