TP53 and pancreatic ductal adenocarcinoma: Given the remarkable benefits of cancer modeling mediated by in vivo gene mutation on large animals with a human-closer competent immune system compared with rodents, primary and metastatic pancreatic ductal adenocarcinoma (PDAC) pig model was developed by delivering TP53-sgRNA, LKB1-sgRNA, and mutant human KRAS gene into the adult pancreases of DIC pigs and following Dox induction.