HOTAIR is a long non-coding RNA that is specifically highly expressed in GBM.833 In addition, HOTAIR expression upregulates with increasing malignancy of GBM.834 The HOTAIR distribution and content in the brain could be observed by SPECT through intravenous injection of 99mTc-conjugated ASO against HOTAIR, thus providing valid diagnostic information for GBM.835 The pharmacokinetics and pharmacodynamics of nucleic acid drugs can be accurately detected and evaluated by in vivo SPECT, PET-based multi-modal imaging. The gene discussed is HOTAIR; the disease is glioblastoma.