To further confirm that the enhanced parthanatos in MLKL-KO cells was independent of its well-defined role in necroptosis, MLKL mutant (T357A/S358A, A/A) that could not be activated by RIPK3, and the phosphomimic mutant (T357E/S358D, E/D) were overexpressed in MLKL-KO HCC cells. The gene discussed is RIPK3; the disease is hepatocellular carcinoma.