By analyzing multispectral images stained with Siglec-15, PAN-CK, and CD68, we divided ESCC samples into 4 groups according to their Siglec-15 expression patterns (eFigure 4 in Supplement 1): TC Siglec-15 positive and macrophage Siglec-15 positive (pattern 1), TC Siglec-15 negative and macrophage Siglec-15 positive (pattern 2), TC Siglec-15 positive and macrophage Siglec-15 negative (pattern 3), and TC Siglec-15 negative and macrophage Siglec-15 negative (pattern 4). The gene discussed is CD68; the disease is esophageal squamous cell carcinoma.