The RNA-seq data of 169 TCGA-GBM samples were obtained, and the tumor samples were clustered into two groups based on cuproptosis genes (FDX1, LIAS, LIPT1, DLD, DLAT, PDHA1, PDHB, MTF1, GLS, and CDKN2A) through consistent cluster analysis (Fig. 1A, B, C). The gene discussed is GLS; the disease is glioblastoma.