The results demonstrated that aspirin could down-regulate the expression of TLR4 in CRC cells compared with control group (HCT116: 24 h, 0.64 ± 0.03, p = 0.0007, 48 h, 0.37 ± 0.05, p = 0.0003. The gene discussed is TLR4; the disease is colorectal carcinoma.