CT26: 24 h, 0.59 ± 0.08, p = 0.0069, 48 h, 0.38 ± 0.04, p = 0.0002) (Fig. 3A and B), which indicated that aspirin might inhibit the stemness of CRC cells by down-regulating of TLR4 expression. This evidence concerns the gene TLR4 and colorectal carcinoma.