In order to further prove that NE can regulate the expression level of WNT7A, we detected the expression levels of Wnt1, Wnt2, Wnt3a, Wnt4, Wnt5a, Wnt6, Wnt7a and Wnt10a in tumour tissues of anti-PD-1 mAb + Vehicle and NE + anti-PD-1 mAb + Vehicle groups. This evidence concerns the gene WNT3A and neoplasm.