A PPI network analysis revealed that key transcription factors associated with LD-type ICC organoids included ATF2 (activating transcription factor 2) (odds ratio = 1.93; P < 0.0001), ELK1 (ETS transcription factor ELK1) (odds ratio = 2.87; P = 0.0023), CTNNB1 (catenin beta 1) (odds ratio = 1.47; P = 0.0046), FLI1 (friend leukemia integration 1 transcription factor) (odds ratio = 3.97; P = 0.0062), and ZNF217 (zinc finger protein 217) (odds ratio = 4.96; P = 0.0105) (Fig. 4e). This evidence concerns the gene ELK1 and intrahepatic cholangiocarcinoma.