CCNE1 and liver disorder: Wan et al. designed sgRNAs targeting p53 upregulated modulator of apoptosis (PUMA), Cyclin E1 (CcnE1), and K (lysine) acetyltransferase 5 (KAT5), which play important roles in liver disease development, in combination with Cas9 to construct the RNP.217–219 A significant decrease in the expression of all three genes was detected.