We intraperitoneally injected gentamicin (Gent; 2.7 μg/μL, 20 μL per fish), an established nephrotoxin, into zebrafish to induce AKI (Diep et al., 2011; Augusto et al., 1996) and then collected the GFP+/DsRed- kidney cells of Tg(fapb10a:GFP;cdh17:DsRed) zebrafish at 5 days post-injury (5 dpi) by FACS. This evidence concerns the gene CDH17 and acute kidney injury.