Blanchard et al. (2020) (27) developed a BBB model by differentiating isogenic APOE3/3 and APOE4/4 iPSCs into brain ECs, pericyte-like cells, and astrocytes and encapsulating them in Matrigel to form vascular networks and study the effect of the APOE genotype on amyloid accumulation, a common pathological mechanism in AD (28). This evidence concerns the gene APOE and Alzheimer disease.