As expectedly, low risk group was significantly related to poor survival in GSE12417 and the AUC values of survival was larger than 0.67 (Fig. 6C, D).Moreover, we employed other AML-related key genes including NOTCH1, NOTCH2, NOTCH3, NOTCH4, JAGGGED2 and DLL-3, to validate our risk model and got similar results (Fig. 6E, AUC > 0.6). This evidence concerns the gene NOTCH1 and acute myeloid leukemia.