PRUNE2 and carcinoma: We compared gene expression levels (RSEM) in prostatic adenocarcinoma to those in normal prostate samples in the dataset from TCGA (Figure 3A): PCA3 had significantly increased expression in carcinoma (median: 12.4; interquartile range [IQR]: 10.3–13.7) as compared with normal prostate (median: 6.9; IQR: 5.2–9.6, [p-value <0.001]), and PRUNE2 showed simultaneous lower expression in carcinoma (median: 11.4; IQR: 10.7–12.0) versus normal prostate (median: 12.2; IQR: 11.8–12.6, [p-value <0.001]).