As depicted in Figure 3B, comparing tumor gene expression across different prostate cancer pathological grades, PCA3 expression was significantly lower in tumors with Gleason Score greater than 7 (median: 11.6; IQR: 8.4–13.4) than in tumors with Gleason Score 7 (median: 12.8; IQR: 11.3–13.8, [p-value <0.001]) or less than 7 (median: 12.5; IQR: 11.8–13.7, [p-value = 0.01]). This evidence concerns the gene PCA3 and Familial prostate cancer.