Knockdown of RNF128 in colorectal cancer cells followed by the application of the YAP inhibitor verteporfin and the YAP agonist PY-60 resulted in changes in the expression levels of target genes downstream of the Hippo signaling pathway, suggesting that RNF128 could regulate its downstream target genes through the Hippo signaling pathway, which consequently affects the malignant tumor behaviors of colorectal cancer cells. This evidence concerns the gene RNF128 and colorectal cancer.