Western blot and RT-PCR experiments also suggested that TRIM58 silencing tend to increase the stem-like cellular phenotype of these tumor cells, as both protein and mRNA expression of stem-cell marker ALDH1 and CD44 demonstrated notably increase in tumor cells treated by TRIM58 shRNAs (Figures 3(g) and 3(h)). This evidence concerns the gene ALDH1A1 and neoplasm.